Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

Koichi Toyokura; Tatsuaki Goh; Hidefumi Shinohara; Akinori Shinoda; Yuki Kondo; Yoshie Okamoto; Takeo Uehara; Koichi Fujimoto; Yoko Okushima; Yoshifumi Ikeyama; Keiji Nakajima; Tetsuro Mimura; Masao Tasaka; Yoshikatsu Matsubayashi; Hidehiro Fukaki

doi:10.1016/j.devcel.2018.11.031

Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

Dev Cell. 2019 Jan 7;48(1):64-75.e5. doi: 10.1016/j.devcel.2018.11.031. Epub 2018 Dec 20.

Authors

Affiliations

¹ Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
² Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan; Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
³ Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.
⁴ Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan.
⁵ Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
⁶ Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan; Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan.
⁷ Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
⁸ Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
⁹ Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan. Electronic address: [email protected].

PMID: 30581155
DOI: 10.1016/j.devcel.2018.11.031

Abstract

In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.

Keywords: Arabidopsis thaliana; LBD16; PUCHI; RLK7; TOLS2/PIPL3; auxin; lateral root; peptide hormone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Cell Communication / physiology
Cell Differentiation / physiology
Cell Division / physiology
Gene Expression Regulation, Plant / physiology*
Indoleacetic Acids / metabolism
Organogenesis, Plant / physiology
Plant Roots / metabolism*
Plants, Genetically Modified / metabolism

Substances

Arabidopsis Proteins
Indoleacetic Acids