Nested Polymerase Chain Reaction with Specific Primers for Mucorales in the Serum of Patients with Hematological Malignancies

Jpn J Infect Dis. 2019 May 23;72(3):196-198. doi: 10.7883/yoken.JJID.2018.379. Epub 2018 Dec 25.

Abstract

Mucormycosis is an opportunistic infection occurring in immunocompromised hosts with hematological malignancies. Mortality due to mucormycosis in patients with hematological malignancies is high. However, the clinical symptoms of mucormycosis are poorly characterized, and diagnosis is difficult due to the lack of specific culture or serological markers or antigens. We present two cases in which nested polymerase chain reaction with specific primers was used in the serum of patients with hematological malignancies.

Keywords: Mucorales; mucormycosis; nested polymerase chain reaction (nested PCR); opportunistic infection; serum.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • DNA Primers
  • Fatal Outcome
  • Female
  • Hematologic Neoplasms / blood
  • Hematologic Neoplasms / microbiology*
  • Humans
  • Male
  • Mucorales / genetics
  • Mucorales / isolation & purification*
  • Mucormycosis / complications*
  • Opportunistic Infections
  • Polymerase Chain Reaction

Substances

  • DNA Primers