Abstract
Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.
Keywords:
Anoikis; Cannibalism; Cell-in-cell; Engulfment; Entosis; Soft agar; Time-lapse.
MeSH terms
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Amides / pharmacology
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Autophagy-Related Proteins / metabolism*
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Cell Line, Tumor
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Entosis / drug effects
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Entosis / physiology*
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Fluorescent Dyes / chemistry
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Humans
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Intravital Microscopy / instrumentation
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Intravital Microscopy / methods*
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Lipid Metabolism / physiology
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Lysosomes / metabolism
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods
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Microtubule-Associated Proteins / metabolism*
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Neoplasms / pathology*
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Pyridines / pharmacology
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Time-Lapse Imaging / instrumentation
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Time-Lapse Imaging / methods
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Vacuoles / metabolism
Substances
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Amides
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Autophagy-Related Proteins
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Fluorescent Dyes
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MAP1LC3A protein, human
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Microtubule-Associated Proteins
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Pyridines
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Y 27632