Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient

Elena Daoutsali; Ronald A M Buijsen; Simone van de Pas; Anke 't Jong; Harald Mikkers; Tom Brands; Bert Eussen; Annelies de Klein; Linda M van der Graaf; Barry A Pepers; Christian Freund; Gisela M Terwindt; Valeria V Orlova; Willeke M C van Roon-Mom

doi:10.1016/j.scr.2018.101359

Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient

Stem Cell Res. 2019 Jan:34:101359. doi: 10.1016/j.scr.2018.101359. Epub 2018 Dec 14.

Affiliations

¹ Department of Human Genetics, LUMC, Leiden, The Netherlands. Electronic address: [email protected].
² Department of Human Genetics, LUMC, Leiden, The Netherlands.
³ LUMC hiPSC Core Facility, Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands.
⁴ Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands; Department of Ophthalmology, Erasmus MC, Rotterdam, The Netherlands.
⁵ Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands.
⁶ Department of Neurology, LUMC, Leiden, The Netherlands.
⁷ Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands.

PMID: 30611017
DOI: 10.1016/j.scr.2018.101359

Abstract

Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type (HCHWA-D) is an autosomal dominant hereditary disease caused by a point mutation in exon 17 of the APP gene. We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic HCHWA-D patient by using non-integrating Sendai virus (SeV). The newly generated hiPSCs express all pluripotency markers, have a normal karyotype, carry the Dutch mutation, can differentiate in the three germ layers in vitro and are SeV free.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cell Culture Techniques / methods*
Cell Line
Cerebral Amyloid Angiopathy, Familial / pathology*
Female
Humans
Induced Pluripotent Stem Cells / pathology*
Middle Aged

Supplementary concepts

Amyloidosis, Cerebroarterial, App-Related