Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

Ruibin Jiang; Yan Guo; Nipi Chen; Chengxian Gao; Zhishan Ding; Bo Jin

doi:10.1155/2018/8617314

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

Evid Based Complement Alternat Med. 2018 Dec 5:2018:8617314. doi: 10.1155/2018/8617314. eCollection 2018.

Authors

Ruibin Jiang^#^{1

2}, Yan Guo^#¹, Nipi Chen¹, Chengxian Gao¹, Zhishan Ding³, Bo Jin¹

Affiliations

¹ College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
² Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.
³ College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China.

^# Contributed equally.

Abstract

This study aimed to investigate whether the total flavonoids (TFs) from Carya cathayensis Sarg. leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms. H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment. The results indicated that TFs significantly alleviate H/R injury, which include inhibiting apoptosis and enhancing antioxidant capacity. The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. Conversely, blocking miR-21 expression with miR-21 inhibitor effectively suppressed the protective effects of TFs against H/R-induced injury. Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax.