Crystallization and preliminary X-ray diffraction studies of 3-methyladenine-DNA glycosylase II from Escherichia coli

Y Yamagata; K Odawara; K Tomita; Y Nakabeppu; M Sekiguchi

doi:10.1016/0022-2836(88)90063-0

Crystallization and preliminary X-ray diffraction studies of 3-methyladenine-DNA glycosylase II from Escherichia coli

J Mol Biol. 1988 Dec 20;204(4):1055-6. doi: 10.1016/0022-2836(88)90063-0.

Authors

Y Yamagata¹, K Odawara, K Tomita, Y Nakabeppu, M Sekiguchi

Affiliation

¹ Faculty of Pharmaceutical Sciences, Osaka University, Suita, Japan.

PMID: 3065516
DOI: 10.1016/0022-2836(88)90063-0

Abstract

Single crystals of 3-methyladenine-DNA glycosylase II produced from the alkA gene of Escherichia coli have been obtained by the method of vapour diffusion with polyethylene glycol 6000 as precipitant at pH 8.5. The crystals belong to the monoclinic space group C2, with a = 58.6 A, b = 76.8 A, c = 62.2 A, beta = 110.3 degrees. They contain one molecule per asymmetric unit and diffract to 2.7 A resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
DNA Glycosylases*
Escherichia coli / enzymology*
N-Glycosyl Hydrolases*
X-Ray Diffraction

Substances

3-methyladenine-DNA glycosylase
DNA Glycosylases
N-Glycosyl Hydrolases