Abstract
Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.
Keywords:
B16 cells; Bone metastasis; Firefly luciferase; Imaging; Melanoma.
MeSH terms
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Animals
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Bone Neoplasms / diagnostic imaging
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Bone Neoplasms / pathology
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Bone Neoplasms / secondary*
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Line, Tumor / transplantation
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Humans
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Luciferases / chemistry
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Luminescent Measurements / instrumentation
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Luminescent Measurements / methods
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Melanoma, Experimental / pathology*
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Mice
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Mice, Inbred C57BL
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Optical Imaging / instrumentation
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Optical Imaging / methods
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Skin Neoplasms / pathology*
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Tibia / diagnostic imaging
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Tibia / pathology