Macrophage cell tracking PET imaging using mesoporous silica nanoparticles via in vivo bioorthogonal F-18 labeling

Hyeon Jin Jeong; Ran Ji Yoo; Jin Kwan Kim; Min Hwan Kim; Su Hong Park; Haebin Kim; Jae Wook Lim; Sun Hee Do; Kyo Chul Lee; Yong Jin Lee; Dong Wook Kim

doi:10.1016/j.biomaterials.2019.01.043

Macrophage cell tracking PET imaging using mesoporous silica nanoparticles via in vivo bioorthogonal F-18 labeling

Biomaterials. 2019 Apr:199:32-39. doi: 10.1016/j.biomaterials.2019.01.043. Epub 2019 Feb 2.

Authors

Affiliations

¹ Department of Chemistry and Chemical Engineering, Inha University, 100 Inharo, Namgu, Incheon, 22212, South Korea.
² Department Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-706, South Korea.
³ Department of Clinical Pathology, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Seoul, South Korea.
⁴ Department Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-706, South Korea. Electronic address: [email protected].
⁵ Department of Chemistry and Chemical Engineering, Inha University, 100 Inharo, Namgu, Incheon, 22212, South Korea. Electronic address: [email protected].

PMID: 30735894
DOI: 10.1016/j.biomaterials.2019.01.043

Abstract

We introduce an efficient cell tracking imaging protocol using positron emission tomography (PET). Since macrophages are known to home and accumulate in tumor tissues and atherosclerotic plaque, we design a PET imaging protocol for macrophage cell tracking using aza-dibenzocyclooctyne-tethered PEGylated mesoporous silica nanoparticles (DBCO-MSNs) with the short half-life F-18-labeled azide-radiotracer via an in vivo strain-promoted alkyne azide cycloaddition (SPAAC) covalent labeling reaction inside macrophage cells in vivo. This PET imaging protocol for in vivo cell tracking successfully visualizes the migration of macrophage cells into the tumor site by the bioorthogonal SPAAC reaction of DBCO-MSNs with [¹⁸F]fluoropentaethylene glycolic azide ([¹⁸F]2) to form ¹⁸F-labeled aza-dibenzocycloocta-triazolic MSNs (¹⁸F-DBCOT-MSNs) inside RAW 264.7 cells. The tissue radioactivity distribution results were consistent with PET imaging findings. In addition, PET images of atherosclerosis in ApoE^-/- mice fed a western diet for 30 weeks were obtained using the devised macrophage cell-tracking protocol.

Keywords: Bioothogonal labeling; Cell tracking; Fluorine-18; Macrophage cells; Mesoporous silica nanoparticles; PET molecular imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / metabolism
Antigens, Differentiation, Myelomonocytic / metabolism
Apolipoproteins E / deficiency
Apolipoproteins E / metabolism
Atherosclerosis / diagnostic imaging
Atherosclerosis / pathology
Aza Compounds / chemical synthesis
Aza Compounds / chemistry
Cell Line, Tumor
Cell Tracking*
Cyclooctanes / chemical synthesis
Cyclooctanes / chemistry
Fluorine Radioisotopes / chemistry*
Humans
Macrophages / cytology*
Mice
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Phagocytosis
Porosity
Positron-Emission Tomography*
RAW 264.7 Cells
Silicon Dioxide / chemistry*
Staining and Labeling*

Substances

(18F)azadibenzocyclooctyne
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Apolipoproteins E
Aza Compounds
CD68 antigen, human
Cyclooctanes
Fluorine Radioisotopes
Silicon Dioxide
Fluorine-18