Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil

Viruses. 2019 Feb 10;11(2):147. doi: 10.3390/v11020147.

Abstract

Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.

Keywords: Aedes aegypti; Amazon forest; Brazil; Culex quinquefasciatus; Hubei reo-like virus 7; RNA-dependent RNA polymerase; arbovirus; birds; insect-viruses; metagenomic; mosquitoes; reovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / virology*
  • Animals
  • Brazil
  • Culex / virology*
  • Female
  • High-Throughput Nucleotide Sequencing
  • Metagenomics
  • Orthoreovirus, Mammalian / enzymology*
  • Orthoreovirus, Mammalian / genetics*
  • RNA, Viral / genetics
  • RNA-Dependent RNA Polymerase / genetics*
  • Rainforest
  • Reoviridae Infections

Substances

  • RNA, Viral
  • RNA-Dependent RNA Polymerase