A systematic genetic screen identifies essential factors involved in nuclear size control

PLoS Genet. 2019 Feb 13;15(2):e1007929. doi: 10.1371/journal.pgen.1007929. eCollection 2019 Feb.

Abstract

Nuclear size correlates with cell size, but the mechanism by which this scaling is achieved is not known. Here we screen fission yeast gene deletion mutants to identify essential factors involved in this process. Our screen has identified 25 essential factors that alter nuclear size, and our analysis has implicated RNA processing and LINC complexes in nuclear size control. This study has revealed lower and more extreme higher nuclear size phenotypes and has identified global cellular processes and specific structural nuclear components important for nuclear size control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / genetics*
  • Cell Size
  • Cytoplasm / genetics
  • Cytoskeleton / genetics
  • Gene Deletion
  • Gene Expression Regulation, Fungal / genetics
  • Genetic Testing / methods
  • Phenotype
  • Schizosaccharomyces / genetics