The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans

Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.

Abstract

β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-"omic" analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteroides / genetics
  • Bacteroides / metabolism
  • Clostridiales / enzymology
  • Clostridiales / genetics
  • Clostridiales / metabolism*
  • Diet
  • Dietary Carbohydrates / metabolism*
  • Gastrointestinal Microbiome
  • Humans
  • Male
  • Mannans / metabolism*
  • Mice

Substances

  • Dietary Carbohydrates
  • Mannans