A Polymer Coating Transfer Enrichment Method for Direct Mass Spectrometry Analysis of Lipids in Biofluid Samples

Angew Chem Int Ed Engl. 2019 Apr 23;58(18):6064-6069. doi: 10.1002/anie.201900011. Epub 2019 Mar 27.

Abstract

A porous polymer coating transfer enrichment method is developed for the direct mass spectrometry (MS) analysis of lipids. The enrichment is fast (ca. 1 min) and enables the profiling and quantitation of lipids in small-volume biofluid samples. Coupled with a photochemical Paternò-Büchi reaction, this method enables the fast determination of lipid structure at the C=C location level and point-of-care lipid biomarker analysis.

Keywords: analyte enrichment; biofluids; lipid isomers; mass spectrometry; polymer coatings.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Lipids / chemistry*
  • Molecular Structure
  • Polymers / chemistry*
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Lipids
  • Polymers