Abstract
Certain proteases are involved in Alzheimer's disease (AD) and their erroneous control may contribute to the pathology onset and progression. In this study we evaluated the cerebral expression of eight proteases, involved in both AβPP processing and extracellular matrix remodeling. Among these proteases, ADAM10, ADAMTS1, Cathepsin D, and Meprin β show a significantly higher mRNAs expression in sporadic AD subjects versus controls, while ADAMTS1, Cathepsin D, and Meprin β show an increment also at the protein level. These data indicate that transcriptional events affecting brain proteases are activated in AD patients, suggesting a link between proteolysis and AD.
Keywords:
ADAMTS1; Alzheimer’s disease; Cathepsin D; Meprin β; RT-PCR; cancer; western blot; α-secretase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADAM10 Protein / metabolism
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ADAM17 Protein / metabolism
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Aged
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Aged, 80 and over
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Alzheimer Disease / enzymology*
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Amyloid Precursor Protein Secretases / metabolism
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Amyloid beta-Protein Precursor / metabolism
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Blotting, Western
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Brain / enzymology*
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Case-Control Studies
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Cathepsin D / metabolism
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Cathepsin L / metabolism
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Female
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Humans
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Male
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Matrix Metalloproteinase 9 / metabolism
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Membrane Proteins / metabolism
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Metalloendopeptidases / metabolism
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Middle Aged
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Peptide Hydrolases / metabolism*
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Real-Time Polymerase Chain Reaction
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Up-Regulation
Substances
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Amyloid beta-Protein Precursor
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Membrane Proteins
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Amyloid Precursor Protein Secretases
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Peptide Hydrolases
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CTSL protein, human
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Cathepsin L
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CTSD protein, human
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Cathepsin D
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Metalloendopeptidases
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meprin A
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MMP9 protein, human
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Matrix Metalloproteinase 9
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ADAM10 Protein
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ADAM10 protein, human
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ADAM17 Protein
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ADAM17 protein, human