Antigen-specific functions of a CD4+ subset of human T lymphocytes with granular morphology

J Immunol. 1986 Mar 15;136(6):2095-102.

Abstract

We previously showed that a small proportion of the CD4+ human lymphocytes express the C3bi receptor (CD11). In the present investigation these cells were found to have the homogeneous morphology of granular lymphocytes. Between 70 and 80% of the cells reacted with antibody Leu-7, but they did not express Fc receptors and had no spontaneous cytotoxic activity against the NK-sensitive cell line K562. The CD11+/CD4+ cells uniformly expressed CD3 and could be induced to express IL 2 receptors by activation with PHA or anti-CD3 antibody. Compared with unfractionated T cells, however, CD11+/CD4+ granular lymphocytes had a reduced ability to produce IL 2 after stimulation with PHA or anti-CD3 antibody. Nevertheless, the CD11+/CD4+ subset generated specific allocytotoxicity after stimulation with allogeneic cells and culture in IL 2. Furthermore, the CD11+/CD4+ cells also proliferated after stimulation with tetanus toxoid and PPD. Thus some cells of the CD11+/CD4+ subset are capable of antigen-specific responses. These results indicate that certain CD11+ cells with granular morphology are mature T lymphocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / analysis*
  • Cell Differentiation
  • Concanavalin A
  • Humans
  • Interleukin-2 / immunology
  • Isoantibodies
  • Isoantigens
  • Killer Cells, Natural / immunology
  • Lymphocyte Activation
  • Microscopy, Electron
  • Phytohemagglutinins
  • Receptors, Immunologic / analysis
  • Receptors, Interleukin-2
  • T-Lymphocytes / classification
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • Tetanus Toxoid / immunology
  • Tuberculin / immunology

Substances

  • Antigens
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Interleukin-2
  • Isoantibodies
  • Isoantigens
  • Phytohemagglutinins
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Tetanus Toxoid
  • Tuberculin
  • Concanavalin A