Haematological and immunophenotypic evaluation of peripheral blood cells of cattle naturally infected with bovine papillomavirus

Paula B Bassi; Fernanda F Araujo; Guilherme C Garcia; Matheus F Costa E Silva; Eustaquio R Bittar; Candice M Bertonha; Olindo A Martins-Filho; Marcio Sobreira Silva Araujo; Joely F Bittar

doi:10.1016/j.tvjl.2018.12.004

Haematological and immunophenotypic evaluation of peripheral blood cells of cattle naturally infected with bovine papillomavirus

Vet J. 2019 Feb:244:112-115. doi: 10.1016/j.tvjl.2018.12.004. Epub 2018 Dec 21.

Authors

Paula B Bassi¹, Fernanda F Araujo², Guilherme C Garcia¹, Matheus F Costa E Silva², Eustaquio R Bittar¹, Candice M Bertonha¹, Olindo A Martins-Filho³, Marcio Sobreira Silva Araujo⁴, Joely F Bittar¹

Affiliations

¹ Universidade de Uberaba (UNIUBE), Medicina Veterinária, Mestrado em Sanidade e Produção Animal nos Trópicos - Avenida Nenê Sabino 1697/1698, 38055-500, Uberaba-MG, Brazil.
² Universidade de Uberaba (UNIUBE), Medicina Veterinária, Mestrado em Sanidade e Produção Animal nos Trópicos - Avenida Nenê Sabino 1697/1698, 38055-500, Uberaba-MG, Brazil.; Grupo Integrado de Pesquisa em Biomarcadores, Instituto René Rachou - Fundação Oswaldo Cruz. Avenida Augusto de Lima nº 1715, 30190-002, Barro Preto, Belo Horizonte-MG, Brazil.
³ Grupo Integrado de Pesquisa em Biomarcadores, Instituto René Rachou - Fundação Oswaldo Cruz. Avenida Augusto de Lima nº 1715, 30190-002, Barro Preto, Belo Horizonte-MG, Brazil.
⁴ Universidade de Uberaba (UNIUBE), Medicina Veterinária, Mestrado em Sanidade e Produção Animal nos Trópicos - Avenida Nenê Sabino 1697/1698, 38055-500, Uberaba-MG, Brazil.; Grupo Integrado de Pesquisa em Biomarcadores, Instituto René Rachou - Fundação Oswaldo Cruz. Avenida Augusto de Lima nº 1715, 30190-002, Barro Preto, Belo Horizonte-MG, Brazil.. Electronic address: [email protected].

PMID: 30825886
DOI: 10.1016/j.tvjl.2018.12.004

Abstract

Papillomaviruses are among the most widespread animal viruses, with many hosts harbouring multiple virus types. The present study aimed to evaluate the haematological and immunophenotypic profile of cattle infected with bovine papillomavirus (BPV). Blood samples were collected from 10 animals with clinical cutaneous BPV and without clinical papillomatosis (control). Haematological analysis demonstrated a significant reduction in haemoglobin and haematocrit for BPV-infected animals. The results also showed an increase of natural killer cells and a decrease of γδ⁺ T-cells and the CD4⁺/CD8⁺ ratio for the BPV group when compared to the control group. The infection was also found to stimulate a pro-inflammatory profile with the participation of CD8⁺T cells producing elevated IFN-γ and IL-17. These findings, although preliminary, provide a better understanding of the immune response of cattle infected with BPV.

Keywords: Cattle; Cytokines; Immune response; Papillomavirus.

MeSH terms

Animals
Bovine papillomavirus 1 / isolation & purification*
Bovine papillomavirus 1 / pathogenicity
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Case-Control Studies
Cattle
Cattle Diseases / blood
Cattle Diseases / immunology*
Cattle Diseases / virology
Female
Immunophenotyping / veterinary
Killer Cells, Natural
Male
Papillomavirus Infections / immunology
Papillomavirus Infections / veterinary*
Papillomavirus Infections / virology