High genetic diversity of noroviruses in children from a community-based study in Rio de Janeiro, Brazil, 2014-2018

Arch Virol. 2019 May;164(5):1427-1432. doi: 10.1007/s00705-019-04195-z.

Abstract

We report on the occurrence and diversity of noroviruses in children (younger than 5 years old of age) from a low-income urban area in Rio de Janeiro, Brazil. Sixty-one stool specimens collected from children between 1 and 4 years old with acute diarrhoeic episodes (ADE) and non-ADE were investigated. RT-qPCR and sequencing of PCR products after conventional RT-PCR analysis were performed. Noroviruses were detected in 29 (47.5%) samples: 21 (46.7%) from cases with ADE and 8 (50%) from non-ADE cases. Molecular characterization showed 10 different genotypes circulating in this community between November 2014 and April 2018.

MeSH terms

  • Brazil
  • Child, Preschool
  • Feces / virology
  • Gastroenteritis / diagnosis
  • Gastroenteritis / virology*
  • Genetic Variation / genetics*
  • Genotype
  • Humans
  • Infant
  • Norovirus / genetics*
  • Norovirus / isolation & purification
  • Phylogeny
  • Poverty
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, DNA