Abstract
Little is known about the molecular and cellular mechanisms involved in the formation of the cranial peripheral sensory system in vertebrates. To identify genes involved in the formation of these circuits, we performed a forward genetic screen utilizing a transgenic zebrafish line (p2rx3.2:gfpsl1) that expresses green fluorescent protein (gfp) in sensory neurons of the Vth, VIIth, IXth and Xth cranial ganglia. Here, we describe a novel zebrafish mutant in which a missense mutation in the adam19b gene selectively affects the epibranchial sensory circuits.
Keywords:
axon guidance; cranial nerves; metalloprotease; sensory circuits; zebrafish.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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ADAM Proteins / genetics
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ADAM Proteins / metabolism*
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Animals
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Animals, Genetically Modified
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Axon Guidance / genetics
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Axon Guidance / physiology*
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CRISPR-Associated Protein 9 / genetics
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CRISPR-Associated Protein 9 / metabolism
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Clustered Regularly Interspaced Short Palindromic Repeats
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DNA Mutational Analysis
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Embryo, Nonmammalian
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Ganglia, Invertebrate / cytology
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Gene Expression Regulation, Developmental / physiology
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Larva
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Mutation / genetics
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Receptors, Purinergic P2 / genetics
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Receptors, Purinergic P2 / metabolism
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Rhombencephalon / cytology*
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Rhombencephalon / physiology*
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Sensory Receptor Cells / metabolism*
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Zebrafish
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Zebrafish Proteins / genetics
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Zebrafish Proteins / metabolism
Substances
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Receptors, Purinergic P2
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Zebrafish Proteins
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p2rx3a protein, zebrafish
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Green Fluorescent Proteins
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CRISPR-Associated Protein 9
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ADAM Proteins