Objectives: To develop a method to screen and quantify 10 common herbicides （paraquat, diquat, glyphosate, glufosinate, cyanazine, atrazine, metazachlor, acetochlor, chlorsulfuron, and metsulfuron） in blood.
Methods: With acetonitrile-water solution [V（acetonitrile）∶V（water）=3∶1] as protein precipitant, 10 common herbicides in blood were detected using ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）.
Results: All the 10 herbicides had good linearity in their linear range （coefficient of determination R2≥0.993）, with the recovery rates 67.4%-111.9%, the relative standard deviations 1.5%-10.8%, the accuracies 85.1%-106.1%, intra-day precisions 2.7%-13.5%, and inter-day precisions 3.3%-13.3%.
Conclusions: This method is easy to operate with high recovery rates. It enables rapid and accurate qualitative screening and quantitative analysis of various herbicides in blood simultaneously.
题目: 血液中常见除草剂的UPLC-HRMS分析.
目的: 建立一种血液中10种常见除草剂（百草枯、敌草快、草甘膦、草铵膦、氰草津、莠去津、吡草胺、乙草胺、氯磺隆、甲磺隆）的筛查及定量方法。.
方法: 以乙腈-水溶液［V（乙腈）∶V（水）=3∶1］为蛋白沉淀剂，用超高效液相色谱-高分辨质谱法（ultra-high performance liquid chromatography-high resolution mass spectrometry，UPLC-HRMS）对血液中的10种除草剂进行检测。.
结果: 10种除草剂在各自线性范围内线性关系良好（决定系数R2≥0.993），回收率为67.4%~111.9%，相对标准偏差为1.5%~10.8%，准确度为85.1%~106.1%，日内精密度为2.7%~13.5%，日间精密度为3.3%~13.3%。.
结论: 该方法操作简便、回收率高，可同时对血液中多种除草剂进行快速准确的定性筛选和定量分析。.
关键词: 法医毒理学；除草剂；超高效液相色谱-高分辨质谱；蛋白沉淀法；生物检材.
Keywords: forensic toxicology; herbicide; ultra-high performance liquid chromatography-high resolution mass spectrometry; protein precipitation; biological samples.
Copyright© by the Editorial Department of Journal of Forensic Medicine.