Draft Genome Sequences of a Ceftazidime-Resistant Acinetobacter baumannii Donor and a Conjugal Escherichia coli Recipient with Acquired Resistance

Hongjie Chen; Xiaoqiong Gu; Charmaine Ng; Laurence Haller; Francis Rathinam Charles; Karina Yew-Hoong Gin

doi:10.1128/MRA.00024-19

Draft Genome Sequences of a Ceftazidime-Resistant Acinetobacter baumannii Donor and a Conjugal Escherichia coli Recipient with Acquired Resistance

Microbiol Resour Announc. 2019 Mar 28;8(13):e00024-19. doi: 10.1128/MRA.00024-19.

Authors

Hongjie Chen¹, Xiaoqiong Gu¹, Charmaine Ng², Laurence Haller¹, Francis Rathinam Charles¹, Karina Yew-Hoong Gin^{3

4}

Affiliations

¹ Department of Civil and Environmental Engineering, National University of Singapore, Singapore.
² Department of Surgery, National University of Singapore, Singapore.
³ Department of Civil and Environmental Engineering, National University of Singapore, Singapore [email protected].
⁴ NUS Environmental Research Institute (NERI), Singapore.

Abstract

A ceftazidime-resistant Acinetobacter baumannii strain was isolated from hospital wastewater and used as the donor in a filter mating experiment with an Escherichia coli strain as the recipient. Recipient, donor, and transconjugant were sequenced, and both donor and transconjugant were found to harbor highly similar plasmid sequences, suggesting that plasmid transfer had occurred.