Activation of PSGR with β-ionone suppresses prostate cancer progression by blocking androgen receptor nuclear translocation

Cancer Lett. 2019 Jul 1:453:193-205. doi: 10.1016/j.canlet.2019.03.044. Epub 2019 Mar 27.

Abstract

The prostate-specific G protein-coupled receptor (PSGR) is a class A G protein-coupled receptor (GPCR) that is specifically expressed in prostate epithelial cells, and its expression has been linked to prostate cancer (PCa) progression. Here, we show that activation of PSGR with its ligand β-ionone, an end-ring analog of β-carotenoid, can suppress PCa cell growth both in vitro and in vivo model. Dissection of the mechanism underlying this relationship reveals that activation of PSGR by β-ionone suppresses AR nuclear translocation via phosphorylation of AR at residue Ser650 by p38 and JNK, which leads to the suppression of AR transactivation, further suppressing PCa cell growth. Overall, we link a cancer cell-specific GPCR with the nuclear AR and show that targeting PSGR can provide us a new target to combat PCa better.

Keywords: Androgen receptor; JNK; PSGR; Prostate cancer; p38.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Nucleus / metabolism
  • Disease Progression
  • Humans
  • MAP Kinase Kinase 4 / metabolism
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / metabolism*
  • Norisoprenoids / pharmacology*
  • PC-3 Cells
  • Phosphorylation
  • Prostatic Neoplasms / drug therapy*
  • Prostatic Neoplasms / metabolism*
  • Receptors, Androgen / biosynthesis
  • Receptors, Androgen / metabolism*
  • Receptors, Odorant / biosynthesis
  • Receptors, Odorant / metabolism*
  • Xenograft Model Antitumor Assays
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • AR protein, human
  • Neoplasm Proteins
  • Norisoprenoids
  • OR51E2 protein, human
  • Receptors, Androgen
  • Receptors, Odorant
  • beta-ionone
  • p38 Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4