A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

Chem Commun (Camb). 2019 Apr 11;55(31):4562-4565. doi: 10.1039/c9cc00261h.

Abstract

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption.

MeSH terms

  • Animals
  • CRISPR-Associated Protein 9 / genetics*
  • Cell Line
  • Endosomes / metabolism
  • Erythrocytes / cytology
  • Erythrocytes / metabolism
  • Flow Cytometry
  • Fluorescent Dyes / chemistry
  • Gene Editing / methods*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Lipids / chemistry*
  • Rabbits

Substances

  • Fluorescent Dyes
  • Lipids
  • Lipofectamine
  • Green Fluorescent Proteins
  • CRISPR-Associated Protein 9