Cancerous phenotypes associated with hypoxia-inducible factors are not influenced by the volatile anesthetic isoflurane in renal cell carcinoma

Chisato Sumi; Yoshiyuki Matsuo; Munenori Kusunoki; Tomohiro Shoji; Takeo Uba; Teppei Iwai; Hidemasa Bono; Kiichi Hirota

doi:10.1371/journal.pone.0215072

Cancerous phenotypes associated with hypoxia-inducible factors are not influenced by the volatile anesthetic isoflurane in renal cell carcinoma

PLoS One. 2019 Apr 15;14(4):e0215072. doi: 10.1371/journal.pone.0215072. eCollection 2019.

Authors

Chisato Sumi¹, Yoshiyuki Matsuo¹, Munenori Kusunoki¹, Tomohiro Shoji¹, Takeo Uba¹, Teppei Iwai¹, Hidemasa Bono², Kiichi Hirota¹

Affiliations

¹ Department of Human Stress Response Science, Institute of Biomedical Science, Kansai Medical University, Hirakata, Japan.
² Database Center for Life Science (DBCLS), Research Organization of Information and Systems (ROIS), Mishima, Japan.

Abstract

The possibility that anesthesia during cancer surgery may affect cancer recurrence, metastasis, and patient prognosis has become one of the most important topics of interest in cancer treatment. For example, the volatile anesthetic isoflurane was reported in several studies to induce hypoxia-inducible factors, and thereby enhance malignant phenotypes in vitro. Indeed, these transcription factors are considered critical regulators of cancer-related hallmarks, including "sustained proliferative signaling, evasion of growth suppressors, resistance to cell death, replicative immortality, angiogenesis, invasion, and metastasis." This study aimed to investigate the impact of isoflurane on the growth and migration of derivatives of the renal cell line RCC4. We indicated that isoflurane treatment did not positively influence cancer cell phenotypes, and that hypoxia-inducible factors (HIFs) maintain hallmark cancer cell phenotypes including gene expressions signature, metabolism, cell proliferation and cell motility. The present results indicate that HIF activity is not influenced by the volatile anesthetic isoflurane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anesthetics, Inhalation / pharmacology*
Carcinoma, Renal Cell / drug therapy
Carcinoma, Renal Cell / metabolism
Carcinoma, Renal Cell / pathology*
Cell Movement / drug effects
Cell Proliferation / drug effects
Gene Expression Regulation, Neoplastic / drug effects*
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / genetics
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
Isoflurane / pharmacology*
Kidney Neoplasms / drug therapy
Kidney Neoplasms / metabolism
Kidney Neoplasms / pathology*
Neovascularization, Pathologic / drug therapy
Phenotype
Tumor Cells, Cultured

Substances

Anesthetics, Inhalation
HIF1A protein, human
Hypoxia-Inducible Factor 1, alpha Subunit
Isoflurane

Associated data

figshare/10.6084/m9.figshare.6571730

Grants and funding

This work was supported by Japan Society for the Promotion of Science KAKENHI, Grants JP26670693 and JP24592336 to K.H and JP18K08874 to T.I., by a research grant from Kansai Medical University Research Consortium to K.H., by a research grant from Katano Kai to K.H., and by a grant from the National Bioscience Database Center of the Japan Science and Technology Agency to H.B.