MicroED data collection with SerialEM

Ultramicroscopy. 2019 Jun:201:77-80. doi: 10.1016/j.ultramic.2019.03.009. Epub 2019 Mar 19.

Abstract

The cryoEM method Microcrystal Electron Diffraction (MicroED) involves transmission electron microscope (TEM) and electron detector working in synchrony to collect electron diffraction data by continuous rotation. We previously reported several protein, peptide, and small molecule structures by MicroED using manual control of the microscope and detector to collect data. Here we present a procedure to automate this process using a script developed for the popular open-source software package SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. Depending on crystal and substrate geometry, more than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.

Keywords: Electron diffraction; MicroED; Microcrystal; SerialEM; Transmission electron microscopy; cryoEM.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy / methods
  • Electrons
  • Microscopy, Electron, Transmission / methods*
  • Peptides / chemistry
  • Proteins / chemistry
  • Software

Substances

  • Peptides
  • Proteins