Mass spectrometry based proteomics for the molecular fingerprinting of Fiano, Greco and Falanghina cultivars

Food Res Int. 2019 Jun:120:26-32. doi: 10.1016/j.foodres.2019.02.020. Epub 2019 Feb 10.

Abstract

The official methodologies used for the identification and comparison of vine cultivars are ampelography and ampelometry. These methodologies are essentially based on qualitative assessments or biometric dependent morphological features of the plant. The heterogeneity of cultivars and consequently the increasing demand for a more detailed product typization, led to the introduction of new methodologies for the varietal characterization. In this scenario, proteomics has already proved to be a very useful discipline for the typization of many kinds of edible products. In this paper, we present a proteomic study carried out on three cultivars of Vitis vinifera peculiar of south Italy (Campania) used for white wine production (Fiano, Greco and Falanghina) by advanced biomolecular mass spectrometry approach. Our data highlight variations in the proteomic profiles during ripening for each cultivar and between analyzed cultivars, thus suggesting a new way to outline the biomolecular signature of vines.

Keywords: High throughput proteomics; LC-MS/MS; Label free proteomics; Molecular characterization; Orbitrap; Typization; Vine cultivars.

MeSH terms

  • Fruit / chemistry*
  • Humans
  • Italy
  • Mass Spectrometry / methods
  • Proteomics / methods
  • Species Specificity
  • Vitis / chemistry*
  • Vitis / classification
  • Wine*