Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study

Biomed Res Int. 2019 Mar 18:2019:8961409. doi: 10.1155/2019/8961409. eCollection 2019.

Abstract

Polycaprolactone (PCL) has attracted great attention for bone regeneration attributed to its cost-efficiency, high toughness, and good processability. However, the relatively low elastic modulus, hydrophobic nature, and insufficient bioactivity of pure PCL limited its wider application for bone regeneration. In the present study, the effects of the addition of boron containing bioactive glass (B-BG) materials on the mechanical properties and biological performance of PCL polymer were investigated with different B-BG contents (0, 10, 20, 30, and 40 wt.%), in order to evaluate the potential applications of B-BG/PCL composites for bone regeneration. The results showed that the B-BG/PCL composites possess better tensile strength, human neutral pH value, and fast degradation as compared to pure PCL polymers. Moreover, the incorporation of B-BG could enhance proliferation, osteogenic differentiation, and angiogenic factor expression for rat bone marrow stromal cells (rBMSCs) as compared to pure PCL polymers. Importantly, the B-BG also promoted the angiogenic differentiation for human umbilical vein endothelial cells (HUVECs). These enhanced effects had a concentration dependence of B-BG content, while 30 wt.% B-BG/PCL composites achieved the greatest stimulatory effect. Therefore the 30 wt.% B-BG/PCL composites have potential applications in bone reconstruction fields.

MeSH terms

  • Angiogenesis Inducing Agents / pharmacology*
  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology*
  • Bone Regeneration / drug effects*
  • Boron / pharmacology*
  • Cell Line
  • Elastic Modulus / drug effects
  • Glass / chemistry*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Materials Testing / methods
  • Mesenchymal Stem Cells / drug effects
  • Osteogenesis / drug effects*
  • Polyesters / chemistry*
  • Polymers / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Tensile Strength / drug effects

Substances

  • Angiogenesis Inducing Agents
  • Biocompatible Materials
  • Polyesters
  • Polymers
  • polycaprolactone
  • Boron