Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells

Nat Biomed Eng. 2018 Mar;2(3):173-182. doi: 10.1038/s41551-018-0200-7. Epub 2018 Mar 5.

Abstract

The large-scale and cost-effective production of quality-controlled human pluripotent stem cells (hPSCs) for use in cell therapy and drug discovery would ideally require a chemically defined xenobiotic-free culture system. Towards the development of such a system, costs associated with the use of recombinant proteins as supplements in basal culture media need to be reduced. Here, we describe a growth-factor-free culture medium that uses just three chemical compounds and a lower number of recombinant proteins than used in commercially available media. We show that the culture medium supports the long-term propagation of hPSCs, as confirmed by karyotype, the expression of pluripotency markers and the capacity to differentiate into cell types derived from the three embryonic germ layers. hPSCs growing in the medium were less dependent on glycolytic pathways than cells grown in medium containing growth factors. Moreover, the medium supported the generation of induced pluripotent stem cells derived from either human dermal fibroblasts or peripheral blood mononuclear cells. Our findings should facilitate the ongoing development of a completely xeno-free, chemically defined, synthetic culture system for hPSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cells, Cultured
  • Culture Media / chemistry
  • Culture Media / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / physiology
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / physiology
  • Pluripotent Stem Cells* / cytology
  • Pluripotent Stem Cells* / physiology

Substances

  • Culture Media
  • Intercellular Signaling Peptides and Proteins