Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Kathirvel Alagesan; Daniel Kolarich

doi:10.1016/j.mex.2019.04.007

Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

MethodsX. 2019 Apr 9:6:773-778. doi: 10.1016/j.mex.2019.04.007. eCollection 2019.

Authors

Kathirvel Alagesan^{1

2

3}, Daniel Kolarich^{1

3

4}

Affiliations

¹ Department of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
² Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
³ Institute for Glycomics, Griffith University, Gold Coast Campus, QLD, 4222, Australia.
⁴ ARC Centre for Nanoscale BioPhotonics, Australia.

Abstract

Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. •Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder.•Reduced the protocol from five days down to one.

Keywords: DNA, deoxyribonucleic acid; ESI, electrospray ionization; Egg yolk powder; Glycopeptide isolation; Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder; MS, mass spectrometry; MS/MS, tandem mass spectrometry; SGP, sialylglycopeptide; Synthetic glycobiology.