The visualization of hERG channels in living cells via a fluorescent probe regulated by the synergy between solvatochromism and molecular rotation based on simple targeting of the group 4-benzylaniline

Zhen Qiao; Qiqi Zhou; Hongyi Zhang; Ningning Wei; Yanru Zhang; KeWei Wang

doi:10.1039/c9cc01735f

The visualization of hERG channels in living cells via a fluorescent probe regulated by the synergy between solvatochromism and molecular rotation based on simple targeting of the group 4-benzylaniline

Chem Commun (Camb). 2019 May 7;55(38):5515-5518. doi: 10.1039/c9cc01735f.

Authors

Zhen Qiao¹, Qiqi Zhou, Hongyi Zhang, Ningning Wei, Yanru Zhang, KeWei Wang

Affiliation

¹ Departments of Pharmacology and Medicinal Chemistry, Qingdao University School of Pharmacy, Qingdao 266021, China. [email protected] [email protected].

PMID: 31020283
DOI: 10.1039/c9cc01735f

Abstract

A highly sensitive fluorescent probe CBH based on solvatochromism and molecular rotation was designed and developed for imaging of hERG channels by employing a novel targeting group 4-benzylaniline. More importantly, CBH has the potential for the quantitative analysis of the hERG channels expressed in tumor cells.

MeSH terms

Aniline Compounds / chemistry*
Animals
Blotting, Western
CHO Cells
Cricetulus
Ether-A-Go-Go Potassium Channels / chemistry
Ether-A-Go-Go Potassium Channels / metabolism*
Fluorescent Dyes
HeLa Cells
Humans
Microscopy, Confocal
Solvents / chemistry*

Substances

Aniline Compounds
Ether-A-Go-Go Potassium Channels
Fluorescent Dyes
KCNH1 protein, human
Solvents
benzylaniline