Examining the Role of Nuclear Receptors During In Vivo Chemical-Mediated Breast Tumorigenesis

Jia Yue Shi; Rachel E Rubino; Christopher J B Nicol

doi:10.1007/978-1-4939-9195-2_16

Examining the Role of Nuclear Receptors During In Vivo Chemical-Mediated Breast Tumorigenesis

Methods Mol Biol. 2019:1966:203-210. doi: 10.1007/978-1-4939-9195-2_16.

Authors

Jia Yue Shi¹, Rachel E Rubino², Christopher J B Nicol^{3

4

5}

Affiliations

¹ Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
² Division of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada.
³ Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. [email protected].
⁴ Division of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada. [email protected].
⁵ Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada. [email protected].

PMID: 31041749
DOI: 10.1007/978-1-4939-9195-2_16

Abstract

The chemical carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) has been used for many decades to induce skin, mammary, and ovarian tumors in mice. There are however a wide range of doses and treatment regimens in the literature that sometimes confound comparative interpretations of different studies. Here we describe a proven method to generate in vivo DMBA-mediated murine mammary tumors to enable consistent studies of the cell targeted role of genes of interest during this process.

Keywords: 7,12-Dimethylbenzyl[a]anthracene; Breast tumorigenesis; Chemical carcinogenesis; Nuclear receptors; Peroxisome proliferator-activated receptors.

MeSH terms

9,10-Dimethyl-1,2-benzanthracene / toxicity*
Animals
Breast Neoplasms / chemically induced
Breast Neoplasms / metabolism*
Carcinogenicity Tests / methods*
Carcinogens / toxicity
Female
Mammary Neoplasms, Experimental / chemically induced
Mammary Neoplasms, Experimental / metabolism*
Mice
Receptors, Cytoplasmic and Nuclear / metabolism*

Substances

Carcinogens
Receptors, Cytoplasmic and Nuclear
9,10-Dimethyl-1,2-benzanthracene