Numb confers to inhibit epithelial mesenchymal transition via β-catenin/Lin28 signaling pathway in breast cancer

Exp Mol Pathol. 2019 Aug:109:104262. doi: 10.1016/j.yexmp.2019.104262. Epub 2019 May 13.

Abstract

Objectives: This study aimed to investigate role of Numb in the epithelial mesenchymal transition (EMT) of breast cancer.

Methods: Numb and β-catenin were inhibited in MCF-7 cells using sh-RNA and overexpressed in T47D cells by pcDNA3.0-Numb, pcDNA3.0-β-catenin. Cell proliferation, invasion and migration were evaluated using MTT and Transwell assay, respectively. β-catenin, Lin28, and EMT related markers were determined using qRT-PCR and Western Blotting.

Results: Knockdown of Numb significantly promoted the proliferation, invasion and migration of MCF-7 cells, further increased the expression of β-catenin, Lin28, Snail-1, and N-cadherin, as well as decreased E-cadherin. In T47D cells transfected with pcDNA3.0-Numb, the results were quite the reverse.

Conclusions: Knockdown of Numb could promote the EMT of breast cancer cells via β-cateni/Lin28 signaling pathway.

Keywords: Breast cancer; EMT; Invasion; Migration; Numb; Proliferation.

MeSH terms

  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Cadherins / genetics
  • Cadherins / metabolism
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Epithelial-Mesenchymal Transition / genetics
  • Female
  • Humans
  • MCF-7 Cells
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • RNA Interference
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Signal Transduction / genetics
  • beta Catenin / genetics
  • beta Catenin / metabolism*

Substances

  • Cadherins
  • Lin28A protein, human
  • Membrane Proteins
  • Nerve Tissue Proteins
  • NUMB protein, human
  • RNA-Binding Proteins
  • beta Catenin