Two DevBCA-like ABC transporters are involved in the multidrug resistance of the cyanobacterium Anabaena sp. PCC 7120

FEBS Lett. 2019 Jul;593(14):1818-1826. doi: 10.1002/1873-3468.13450. Epub 2019 Jun 4.

Abstract

The filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 is an important model organism for studying cell differentiation, nitrogen fixation, and photosynthesis. This cyanobacterium possesses a high number of membrane transporters. Not much is known about the roles of the membrane transporters, especially the ATP-binding cassette (ABC) transporters, in the multidrug resistance of this cyanobacterium. In the present work, we performed a mutational analysis of the genes alr4280/alr4281/alr4282 and alr3647/alr3648/alr3649 that code for the components of putative ABC exporters and are homologous to the DevBCA heterocyst-specific glycolipid exporter. We show that these genes are essential for resistance to different drugs and are not essential for heterocyst development.

Keywords: ABC transporter; Anabaena sp. PCC 7120; DevB homolog; cyanobacteria; multidrug resistance; tripartite efflux pump.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Anabaena / drug effects*
  • Anabaena / genetics*
  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Multiple / genetics*
  • Multigene Family / genetics
  • Mutation

Substances

  • ATP-Binding Cassette Transporters
  • Anti-Bacterial Agents