A fast responsive chromogenic and near-infrared fluorescence lighting-up probe for visual detection of toxic thiophenol in environmental water and living cells

Talanta. 2019 Aug 15:201:111-118. doi: 10.1016/j.talanta.2019.03.113. Epub 2019 Apr 2.

Abstract

Thiophenols as high toxic environmental pollutants are poisonous for animals and aquatic organisms. Therefore, it is indispensable to monitor thiophenols in the environment. Herein, a novel near-infrared fluorescent probe was developed for the detection of thiophenols, which was easily prepared by one-step coupling of 2,4-dinitrobenzenesulfonyl chloride with Nile blue. The probe showed a significant near infrared (∼675 nm) fluorescence "turn-on" response to thiophenols with some good features including chromogenic reaction, high sensitivity and selectivity, fast response, near-infrared emission along with low detection limit (1.8 nM). The probe was employed to rapidly and visually determine thiophenols in several industrial wastewaters with good recoveries (90-110%). Moreover, this probe has been demonstrated good capability for imaging thiophenol in HeLa cells.

Keywords: Chromogenic; Fluorescent probe; Imaging; Near-infrared; Nile blue; Thiophenols.

MeSH terms

  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / radiation effects
  • Fluorescent Dyes / toxicity
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Infrared Rays
  • Limit of Detection
  • Microscopy, Confocal / methods
  • Models, Chemical
  • Oxazines / chemical synthesis
  • Oxazines / chemistry*
  • Oxazines / radiation effects
  • Oxazines / toxicity
  • Phenols / analysis*
  • Spectrometry, Fluorescence / methods
  • Sulfhydryl Compounds / analysis*
  • Sulfonamides / chemical synthesis
  • Sulfonamides / chemistry*
  • Sulfonamides / radiation effects
  • Sulfonamides / toxicity
  • Wastewater / analysis*
  • Water Pollutants, Chemical / analysis*

Substances

  • Fluorescent Dyes
  • Oxazines
  • Phenols
  • Sulfhydryl Compounds
  • Sulfonamides
  • Waste Water
  • Water Pollutants, Chemical
  • Nile Blue
  • thiophenol