Full genome sequence of a novel potexvirus from Euonymus bungeanus Maxim based on RNA-Seq analysis

Arch Virol. 2019 Aug;164(8):2201-2204. doi: 10.1007/s00705-019-04293-y. Epub 2019 May 23.

Abstract

In July 2018, a yellow mottle leaf disease was observed on the leaves of Euonymus bungeanus Maxim plants in Liaoning Province of China. Typical flexuous filaments (diameter, 13 nm; length, ca. 500 nm) were observed in extracts on the symptomatic leaves. Transcriptome sequencing indicated the presence of a potexvirus in the infected samples. The complete viral genome, determined to be 6,784 nucleotides in length, excluding the poly(A) tail, contains five open reading frames and is most closely related to that of euonymus yellow vein associated virus (EuYVAV, MF078061) (41.0%). Based on the coat protein gene, this virus shares the highest sequence similarity with cymbidium mosaic virus (CyMV, EF125179), with 43.9% nucleotide and 38.8% amino acid sequence identity, respectively. Phylogenetic analysis indicated that the virus clustered with potexviruses and is most closely related to EuYVaV. This virus is a distinct member of the genus Potexvirus, for which the name "euonymus yellow mottle associated virus" (EuYMaV) is proposed.

MeSH terms

  • Amino Acid Sequence / genetics
  • Base Sequence / genetics
  • Capsid Proteins / genetics
  • China
  • Euonymus / virology*
  • Genome, Viral / genetics*
  • Open Reading Frames / genetics
  • Phylogeny
  • Plant Diseases / virology
  • Plant Leaves / virology
  • Plants / virology
  • Potexvirus / genetics*
  • RNA, Viral / genetics*
  • Satellite Viruses / genetics
  • Sequence Analysis, RNA / methods

Substances

  • Capsid Proteins
  • RNA, Viral

Supplementary concepts

  • Cymbidium mosaic virus