Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods

Gurkaran S Sarohia; Erik D Garza; Sanjoy K Bhattacharya

doi:10.1007/978-1-4939-9488-5_5

Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods

Methods Mol Biol. 2019:1996:47-51. doi: 10.1007/978-1-4939-9488-5_5.

Authors

Gurkaran S Sarohia¹, Erik D Garza², Sanjoy K Bhattacharya³

Affiliations

¹ MD Undergraduate Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. [email protected].
² McKnight Research Center, Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
³ Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

PMID: 31127546
DOI: 10.1007/978-1-4939-9488-5_5

Abstract

Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.

Keywords: Cholesterol; Gas chromatography-mass spectrometry (GC-MS); Lipid extraction; Lipidomics; Lipids.

MeSH terms

Animals
Cholesterol / analysis
Cholesterol / isolation & purification
Cholesterol / metabolism*
Gas Chromatography-Mass Spectrometry / methods*
Lipidomics / methods*
Optic Nerve / metabolism*
Reproducibility of Results
Tandem Mass Spectrometry

Substances

Cholesterol