DNA-Encoded Macrocyclic Peptide Library

Zhengrong Zhu; Alex Shaginian; La Shadric C Grady; Christopher P Davie; Kenneth Lind; Sandeep Pal; Praew Thansandote; Graham L Simpson

doi:10.1007/978-1-4939-9504-2_12

DNA-Encoded Macrocyclic Peptide Library

Methods Mol Biol. 2019:2001:273-284. doi: 10.1007/978-1-4939-9504-2_12.

Authors

Zhengrong Zhu¹, Alex Shaginian², La Shadric C Grady², Christopher P Davie², Kenneth Lind², Sandeep Pal³, Praew Thansandote³, Graham L Simpson³

Affiliations

¹ GlaxoSmithKline, Cambridge, MA, USA. [email protected].
² GlaxoSmithKline, Cambridge, MA, USA.
³ GlaxoSmithKline, Stevenage, UK.

PMID: 31134575
DOI: 10.1007/978-1-4939-9504-2_12

Abstract

DNA-encoded library technology (ELT) is a cutting-edge enabling technology platform for drug discovery. Here we describe how to design and synthesize a macrocyclic DNA-encoded library; how to perform selection, sequencing, and data analysis to identify potential active peptides; and how to synthesize off-DNA peptides to confirm activity. This approach provides an effective tool for pharmaceutical research based on peptides.

Keywords: Affinity selection; DNA-encoded library; Macrocyclic peptides.

MeSH terms

DNA / chemistry*
Drug Discovery
High-Throughput Nucleotide Sequencing
Macrocyclic Compounds / chemical synthesis*
Macrocyclic Compounds / chemistry
Peptide Library*
Peptides, Cyclic / chemical synthesis
Peptides, Cyclic / chemistry*
Protein Interaction Domains and Motifs
Small Molecule Libraries / metabolism

Substances

Macrocyclic Compounds
Peptide Library
Peptides, Cyclic
Small Molecule Libraries
DNA