Autophagy metabolically suppresses CD8+ T cell antitumor immunity

Autophagy. 2019 Sep;15(9):1648-1649. doi: 10.1080/15548627.2019.1628545. Epub 2019 Jun 14.

Abstract

Macroautophagy/autophagy is a critical regulator of adaptive T cell immunity and homeostasis. However, the role of T cell autophagy in regulating antitumor immune responses is less clear. In a recent study, we showed that deletion of the essential autophagy genes Atg5, Atg14, or Atg16l1 in host tissues dramatically impairs growth of autophagy-competent syngeneic tumors. We further demonstrated that CD8+ T cells lacking Atg5 acquire an effector memory phenotype and produce more IFNG/IFN-γ (interferon gamma) and TNF/TNF-α (tumor necrosis factor). These phenotypic changes are accompanied by enhanced glucose metabolism that results in alterations in histone methylation, and upregulation of glycolytic and immune response genes. In accordance with this, we observed control of tumor growth in autophagy-competent mice after adoptive transfer with a sub-therapeutic dose of atg5-/- T cells. Collectively, we discovered a unique, cell-autonomous role for T cell autophagy in the metabolic control of antitumor immunity.

Keywords: Antitumor immunity; CD8 T cells; SAM; autophagy; glycolysis; lactate; methylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy / genetics
  • Autophagy / immunology*
  • Autophagy-Related Protein 5 / genetics
  • Autophagy-Related Protein 5 / metabolism
  • Autophagy-Related Proteins / genetics
  • Autophagy-Related Proteins / metabolism
  • Bone Marrow Transplantation
  • CD8-Positive T-Lymphocytes / immunology*
  • Cell Line, Tumor
  • Glucose / metabolism
  • Histones / chemistry
  • Histones / metabolism
  • Interferon-gamma / metabolism
  • Methylation
  • Mice
  • Mice, Knockout
  • Neoplasms / immunology*
  • Tumor Necrosis Factor-alpha / metabolism
  • Vesicular Transport Proteins / genetics
  • Vesicular Transport Proteins / metabolism

Substances

  • Atg14 protein, mouse
  • Atg5 protein, mouse
  • Autophagy-Related Protein 5
  • Autophagy-Related Proteins
  • Histones
  • Tumor Necrosis Factor-alpha
  • Vesicular Transport Proteins
  • Interferon-gamma
  • Glucose

Grants and funding

This work was supported by the Canadian Breast Cancer Foundation [n/a]; BC Cancer Foundation [n/a].