Abstract
We detected constitutive expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in 3 human solid tumors by Northern blot analysis. Two of them were also found to secrete the GM-CSF protein by colony forming unit-culture assay. Southern blot analysis of each tumor DNA showed no gross rearrangement of the GM-CSF gene. This is the first report that demonstrates expression of the GM-CSF gene in solid tumors at the mRNA level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Colony-Forming Units Assay
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Colony-Stimulating Factors / genetics*
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DNA, Neoplasm / genetics
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Gene Expression Regulation
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Genes
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Granulocyte-Macrophage Colony-Stimulating Factor
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Growth Substances / genetics*
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Humans
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Neoplasms / genetics*
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RNA, Messenger / genetics
Substances
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Colony-Stimulating Factors
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DNA, Neoplasm
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Growth Substances
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RNA, Messenger
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Granulocyte-Macrophage Colony-Stimulating Factor