Ginsenoside Rh2 reverses cyclophosphamide-induced immune deficiency by regulating fatty acid metabolism

J Leukoc Biol. 2019 Nov;106(5):1089-1100. doi: 10.1002/JLB.2A0419-117R. Epub 2019 Jun 18.

Abstract

Ginsenoside Rh2 (G-Rh2) has well-established potent antitumor activity; yet, the effects of G-Rh2 on immune and metabolism regulation in cancer treatment, especially non-small cell lung cancer (NSCLC) remain unclear. We showed that G-Rh2 had a synergistic antitumor effect with cyclophosphamide (CY) on mice with NSCLC, and improved the immune deficiency caused by CY. Consistently, G-Rh2 exhibited no inhibitory effect on tumor growth of T cells-deficient nude mice. Furthermore, G-Rh2 treatment triggered the oxidative decomposition of fatty acid (FA), suppressed FA synthesis, increased ketone level, and decreased glucocorticoid (CORT) secretion. G-Rh2 significantly down-regulated the expression of fatty acid synthase (FASN). Of note, in liver-specific FASN knockout mice G-Rh2 failed to show the same immune enhancement effects. Further mechanistic exploration revealed that G-Rh2 suppressed the expression and nuclear translocation of sterol regulatory element binding protein 1 (SREBP-1), and disturbed the SREBP-1-FASN interaction in vitro.

Keywords: ginsenoside Rh2; immune deficiency; metabolism regulation; synergistic treatment.

MeSH terms

  • A549 Cells
  • Animals
  • Carcinoma, Non-Small-Cell Lung / drug therapy
  • Carcinoma, Non-Small-Cell Lung / immunology
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cyclophosphamide
  • Fatty Acid Synthase, Type I / immunology*
  • Fatty Acids / immunology*
  • Ginsenosides
  • Humans
  • Immune Tolerance / drug effects*
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / immunology
  • Lung Neoplasms / pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Sterol Regulatory Element Binding Protein 1 / immunology*
  • Xenograft Model Antitumor Assays

Substances

  • Fatty Acids
  • Ginsenosides
  • SREBF1 protein, human
  • Srebf1 protein, mouse
  • Sterol Regulatory Element Binding Protein 1
  • ginsenoside Rh2
  • Cyclophosphamide
  • FASN protein, human
  • Fasn protein, mouse
  • Fatty Acid Synthase, Type I