BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells

Deepak B Thimiri Govinda Raj; Niamat Ali Khan; Srisaran Venkatachalam; Sivakumar Arumugam

doi:10.1007/7651_2019_249

BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells

Methods Mol Biol. 2020:2125:205-208. doi: 10.1007/7651_2019_249.

Authors

Deepak B Thimiri Govinda Raj^{1

2

3}, Niamat Ali Khan^{4

5}, Srisaran Venkatachalam⁶, Sivakumar Arumugam⁷

Affiliations

¹ Envirotransgene® Bio-solutions Global, Chennai, India. [email protected].
² DTCG®, Chennai, India. [email protected].
³ CSIR, Pretoria, South Africa. [email protected].
⁴ Envirotransgene® Bio-solutions Global, Chennai, India.
⁵ DTCG®, Chennai, India.
⁶ Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India.
⁷ School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamil Nadu, India.

PMID: 31228126
DOI: 10.1007/7651_2019_249

Abstract

Baculovirus expression vector system (BEVS) is an established technology for recombinant protein expression in insect cells. Further, BEVS-mediated gene transduction of mammalian cells (BacMam) is emerging as a technique for high level recombinant protein expression in mammalian cells. Here, we describe generic method in using BEVS as a BacMam for rapid recombinant protein expression in mammalian cells.

Keywords: BEVS; Bac-to-Bac; BacMam.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Baculoviridae / metabolism*
Gene Expression*
HEK293 Cells
Humans
Recombinant Proteins / metabolism*
Transfection / methods*

Substances

Recombinant Proteins