Facile Preparation of Fe3O4/C Nanocomposite and Its Application for Cost-Effective and Sensitive Detection of Tryptophan

Jun Liu; Shuai Dong; Quanguo He; Suchun Yang; Mei Xie; Peihong Deng; Yonghui Xia; Guangli Li

doi:10.3390/biom9060245

Facile Preparation of Fe₃O₄/C Nanocomposite and Its Application for Cost-Effective and Sensitive Detection of Tryptophan

Biomolecules. 2019 Jun 22;9(6):245. doi: 10.3390/biom9060245.

Authors

Jun Liu¹, Shuai Dong², Quanguo He³, Suchun Yang⁴, Mei Xie⁵, Peihong Deng⁶, Yonghui Xia⁷, Guangli Li⁸

Affiliations

¹ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].
² Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].
³ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].
⁴ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].
⁵ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].
⁶ Key Laboratory of Functional Metal-Organic Compounds of Hunan Province; Key Laboratory of functional Organometallic Materials of Hunan Provincial Universities; Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China. [email protected].
⁷ Zhuzhou Institute for Food and Drug Control, Zhuzhou 412000, China. [email protected].
⁸ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China. [email protected].

Abstract

In this study, we reported facile synthesis of Fe₃O₄/C composite and its application for the cost-effective and sensitive determination of tryptophan (Trp) in human serum samples. Fe₃O₄/C composites were prepared by a simple one-pot hydrothermal method followed by a mild calcination procedure, using FeCl₃∙6H₂O as Fe₃O₄ precursor, and glucose as reducing agent and carbon source simultaneously. The Fe₃O₄/C composite modified glassy carbon electrode (Fe₃O₄/C/GCE) was prepared by drop-casting method. The microstructure and morphology of Fe₃O₄/C composite was characterized by powder X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. Due to large specific surface area and synergistic effect from Fe₃O₄ nanoparticles and carbon coating, Fe₃O₄/C composite showed excellent electrocatalytic activity toward the oxidation of Trp. As a result, the proposed Fe₃O₄/C/GCE displayed superior analytical performances toward Trp determination, with two wide detection ranges (1.0-80 μM and 80-800 μM) and a low detection limit (0.26 μM, S/N = 3). Moreover, successful detection of Trp in human serum samples further validate the practicability of the proposed sensor.

Keywords: Fe3O4/C composite; second derivative linear scan voltammetry; tryptophan; voltammetric detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon / chemistry*
Cost-Benefit Analysis*
Electrochemistry / economics
Electrochemistry / instrumentation*
Electrodes
Humans
Limit of Detection*
Magnetite Nanoparticles / chemistry*
Nanocomposites / chemistry*
Temperature
Time Factors
Tryptophan / analysis*
Tryptophan / blood
Tryptophan / chemistry

Substances

Magnetite Nanoparticles
Carbon
Tryptophan