Data on SVCT2 transporter expression and localization in cancer cell lines and tissues

Francisco J Roa; Eduardo Peña; Eveling Inostroza; Kirsty Sotomayor; Mauricio González; Francisco A Gutierrez-Castro; Michelle Maurin; Karen Sweet; Claire Labrousse; Marcell Gatica; Carlos F Aylwin; Pamela Mendoza; Mafalda Maldonado; Carolina Delgado; Jaime Madariaga; Jessica Panes; Tiare Silva-Grecchi; Ilona I Concha; Gustavo Moraga-Cid; Alejandro M Reyes; Carola Muñoz-Montesino; Juan Carlos Vera; Coralia I Rivas

doi:10.1016/j.dib.2019.103972

Data on SVCT2 transporter expression and localization in cancer cell lines and tissues

Data Brief. 2019 May 6:25:103972. doi: 10.1016/j.dib.2019.103972. eCollection 2019 Aug.

Authors

Francisco J Roa¹, Eduardo Peña¹, Eveling Inostroza¹, Kirsty Sotomayor¹, Mauricio González¹, Francisco A Gutierrez-Castro¹, Michelle Maurin¹, Karen Sweet¹, Claire Labrousse¹, Marcell Gatica¹, Carlos F Aylwin¹, Pamela Mendoza¹, Mafalda Maldonado¹, Carolina Delgado², Jaime Madariaga², Jessica Panes³, Tiare Silva-Grecchi³, Ilona I Concha⁴, Gustavo Moraga-Cid³, Alejandro M Reyes⁴, Carola Muñoz-Montesino³, Juan Carlos Vera¹, Coralia I Rivas¹

Affiliations

¹ Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, PO Box 160C, Concepción, Chile.
² Departamento de Especialidades Médicas, Facultad de Medicina, Universidad de Concepción, Barrio Universitario s/n, PO Box 160C, Concepción, Chile.
³ Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, PO Box 160C, Concepción, Chile.
⁴ Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, PO Box 567, Valdivia, Chile.

Abstract

The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.