Survivors of Ebola Virus Disease Develop Polyfunctional Antibody Responses

Bronwyn M Gunn; Vicky Roy; Marcus M Karim; Jessica N Hartnett; Todd J Suscovich; Augustine Goba; Mambu Momoh; John Demby Sandi; Lansana Kanneh; Kristian G Andersen; Jeffrey G Shaffer; John S Schieffelin; Robert F Garry; Donald S Grant; Galit Alter

doi:10.1093/infdis/jiz364

Survivors of Ebola Virus Disease Develop Polyfunctional Antibody Responses

J Infect Dis. 2020 Jan 1;221(1):156-161. doi: 10.1093/infdis/jiz364.

Authors

Bronwyn M Gunn¹, Vicky Roy¹, Marcus M Karim¹, Jessica N Hartnett², Todd J Suscovich¹, Augustine Goba³, Mambu Momoh^{3

4}, John Demby Sandi³, Lansana Kanneh³, Kristian G Andersen^{5

6}, Jeffrey G Shaffer⁷, John S Schieffelin², Robert F Garry², Donald S Grant^{3

8}, Galit Alter¹

Affiliations

¹ Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts.
² Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana.
³ Viral Hemorrhagic Fever Program, Kenema Government Hospital.
⁴ Eastern Polytechnic University, Kenema, Sierra Leone.
⁵ Department of Immunology and Microbiology, Scripps Research Institute.
⁶ Scripps Research Translational Institute, La Jolla, California.
⁷ Department of Biostatistics and Bioinformatics, Tulane School of Public Health and Tropical Medicine, New Orleans, Louisiana.
⁸ Ministry of Health and Sanitation, Freetown, Sierra Leone.

Abstract

Monoclonal antibodies can mediate protection against Ebola virus (EBOV) infection through direct neutralization as well as through the recruitment of innate immune effector functions. However, the antibody functional response following survival of acute EBOV disease has not been well characterized. In this study, serum antibodies from Ebola virus disease (EVD) survivors from Sierra Leone were profiled to capture variation in overall subclass/isotype abundance, neutralizing activity, and innate immune effector functions. Antibodies from EVD survivors exhibited robust innate immune effector functions, mediated primarily by IgG1 and IgA1. In conclusion, development of functional antibodies follows survival of acute EVD.

Keywords: Ebola virus; antibody; innate immune effector function.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Antibodies, Neutralizing / blood*
Antibodies, Viral / blood*
Antigens, Viral / immunology
Hemorrhagic Fever, Ebola / immunology*
Humans
Immunity, Innate*
Immunoglobulin A / blood
Immunoglobulin G / blood*
Phagocytosis
Sierra Leone
Survivors

Substances

Antibodies, Neutralizing
Antibodies, Viral
Antigens, Viral
Immunoglobulin A
Immunoglobulin G

Abstract

Publication types

MeSH terms

Substances

Grants and funding