The tva(A) Gene from Brachyspira hyodysenteriae Confers Decreased Susceptibility to Pleuromutilins and Streptogramin A in Escherichia coli

Antimicrob Agents Chemother. 2019 Aug 23;63(9):e00930-19. doi: 10.1128/AAC.00930-19. Print 2019 Sep.

Abstract

The tva(A) gene suspected to confer resistance to pleuromutilins in Brachyspira hyodysenteriae was tested for functionality in Escherichia coli AG100A and Staphylococcus aureus RN4220. Expression of the cloned tva(A) gene conferred decreased susceptibility to pleuromutilin (P) and streptogramin A (SA) antibiotics in E. coli and had a minor effect in S. aureus The finding provides evidence of the direct association of tva(A) with the PSA resistance phenotype.

Keywords: ABC-F protein; antibiotic resistance; gene expression; pristinamycin IIA; swine dysentery; tiamulin; valnemulin; virginiamycin M1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brachyspira hyodysenteriae / drug effects*
  • Brachyspira hyodysenteriae / genetics*
  • Diterpenes / pharmacology*
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics*
  • Microbial Sensitivity Tests
  • Pleuromutilins
  • Polycyclic Compounds / pharmacology*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics*
  • Streptogramin A / pharmacology*
  • Swine
  • Swine Diseases / microbiology

Substances

  • Diterpenes
  • Polycyclic Compounds
  • Streptogramin A