A Near-infrared Fluorescent Probe of Dicyanoisophorone Derivatives for Selective Detection and Fluorescence Cellular Imaging of Palladium

Anal Sci. 2019 Dec 10;35(12):1305-1310. doi: 10.2116/analsci.19P136. Epub 2019 Jul 12.

Abstract

Palladium (Pd) has been acknowledged to be a rare inner transition metal, which plays a pivotal role in many fields. This article focuses on developing a safe and effective near-infrared fluorescent probe, MW-PD, which would make a great contribution to the detection of palladium residue in drugs, especially trace residues. The fluorescent probe was rationally designed by combining the dicyanoisophorone fluorophore with an allyloxycarbonyl group. Based on the Tsuji-Trost reaction, the probe exhibited high selectivity and sensitivity toward Pd (0) over other common metal ions with a low detection limit (8.0 nM). Moreover, MW-PD showed biocompatibility and was successfully applied to imaging Pd (0) in Hela cells.

Keywords: Dicyanoisophorone; fluorescence probe; live-cell imaging; near-infrared (NIR); palladium.

MeSH terms

  • Cyclohexanones / chemistry*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Infrared Rays*
  • Limit of Detection*
  • Optical Imaging / methods*
  • Palladium / analysis*
  • Palladium / chemistry*
  • Palladium / metabolism

Substances

  • Cyclohexanones
  • Fluorescent Dyes
  • isophorone
  • Palladium