Production and in vivo PET/CT imaging of the theranostic pair 132/135La

Eduardo Aluicio-Sarduy; Reinier Hernandez; Aeli P Olson; Todd E Barnhart; Weibo Cai; Paul A Ellison; Jonathan W Engle

doi:10.1038/s41598-019-47137-0

Production and in vivo PET/CT imaging of the theranostic pair ^132/135La

Sci Rep. 2019 Jul 23;9(1):10658. doi: 10.1038/s41598-019-47137-0.

Authors

Eduardo Aluicio-Sarduy¹, Reinier Hernandez², Aeli P Olson³, Todd E Barnhart³, Weibo Cai², Paul A Ellison³, Jonathan W Engle^{3

2}

Affiliations

¹ Department of Medical Physics, University of Wisconsin-Madison, Madison, WI, 53705, USA. [email protected].
² Department of Radiology, University of Wisconsin-Madison, Madison, WI, 53705, USA.
³ Department of Medical Physics, University of Wisconsin-Madison, Madison, WI, 53705, USA.

Abstract

The present study describes a novel method for the low energy cyclotron production and radiochemical isolation of no-carrier-added ^132/135La³⁺ from bulk ^natBa. This separation strategy combines precipitation and single-column extraction chromatography to afford an overall radiochemical yield (92 ± 2%) and apparent molar activity (22 ± 4 Mbq/nmol) suitable for the radiolabeling of DOTA-conjugated vectors. The produced ^132/135La³⁺ has a radiochemical and radionuclidic purity amenable for ¹³²La/¹³⁵La-based cancer theranostic applications. Longitudinal PET/CT images acquired using the positron-emitting ¹³²La and ex vivo biodistribution data separately corroborated the accumulation of unchelated ^132/135La³⁺ ions in bone and the liver.