Light Scattering to Quantify Protein-Protein Interactions at High Protein Concentrations

Mahlet A Woldeyes; Cesar Calero-Rubio; Eric M Furst; Christopher J Roberts

doi:10.1007/978-1-4939-9678-0_2

Light Scattering to Quantify Protein-Protein Interactions at High Protein Concentrations

Methods Mol Biol. 2019:2039:23-37. doi: 10.1007/978-1-4939-9678-0_2.

Authors

Mahlet A Woldeyes¹, Cesar Calero-Rubio¹, Eric M Furst¹, Christopher J Roberts²

Affiliations

¹ Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
² Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA. [email protected].

PMID: 31342416
DOI: 10.1007/978-1-4939-9678-0_2

Abstract

Static and dynamic (laser) light scattering (SLS and DLS, respectively) can be used to measure the so-called weak or colloidal protein-protein interactions in solution from low to high protein concentrations (c₂). This chapter describes a methodology to measure protein-protein self-interactions using SLS and DLS, with illustrative examples for monoclonal antibody solutions from low to high protein concentrations (c₂ ~ 1-10² g/L).

Keywords: Dynamic light scattering; Hydrodynamic factor; Protein–protein interactions; Static light scattering; Structure factor.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antibodies, Monoclonal / chemistry
Light
Protein Interaction Domains and Motifs / physiology*
Proteins / chemistry*
Proteins / metabolism*
Scattering, Radiation
Solutions / chemistry

Substances

Antibodies, Monoclonal
Proteins
Solutions

Grants and funding

R01 EB006006/EB/NIBIB NIH HHS/United States