Molecular Characterization of Coxsackievirus B5 Isolates from Sewage, Italy 2016-2017

Stefano Fontana; Stefano Fiore; Gabriele Buttinelli; Concetta Amato; Licia Veronesi; Roberta Zoni; Maria Triassi; Francesca Pennino; Giovanni Maurizio Giammanco; Simona De Grazia; Antonella Cicala; Angelo Siragusa; Sabine Gamper; Silvia Spertini; Paolo Castiglia; Andrea Cossu; Cinzia Germinario; Angela Maria Vittoria Larocca; Paola Stefanelli

doi:10.1007/s12560-019-09395-z

Molecular Characterization of Coxsackievirus B5 Isolates from Sewage, Italy 2016-2017

Food Environ Virol. 2019 Dec;11(4):440-445. doi: 10.1007/s12560-019-09395-z. Epub 2019 Jul 26.

Authors

Stefano Fontana¹, Stefano Fiore¹, Gabriele Buttinelli¹, Concetta Amato¹, Licia Veronesi², Roberta Zoni², Maria Triassi³, Francesca Pennino³, Giovanni Maurizio Giammanco⁴, Simona De Grazia⁴, Antonella Cicala⁵, Angelo Siragusa⁵, Sabine Gamper⁶, Silvia Spertini⁶, Paolo Castiglia⁷, Andrea Cossu⁷, Cinzia Germinario⁸, Angela Maria Vittoria Larocca⁸, Paola Stefanelli⁹

Affiliations

¹ Department of Infectious Diseases, Italian National Institute of Health (Istituto Superiore di Sanità, ISS), Viale Regina Elena 299, 00161, Rome, Italy.
² Department of Medicine and Surgery, University of Parma, Parma, Italy.
³ Department of Public Health, University of Naples Federico II, Naples, Italy.
⁴ Department of Health Promotion, Mother and Child Care and Internal Medicine 'G. D'Alessandro', University of Palermo, Palermo, Italy.
⁵ AMAP S.p.A, Palermo, Italy.
⁶ Comprensorio Sanitario di Bolzano, Servizio Igiene e Sanità Pubblica, Bolzano, Italy.
⁷ Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari, Italy.
⁸ Department of Biomedical Science and Human Oncology, University of Bari "Aldo Moro", Bari, Italy.
⁹ Department of Infectious Diseases, Italian National Institute of Health (Istituto Superiore di Sanità, ISS), Viale Regina Elena 299, 00161, Rome, Italy. [email protected].

Abstract

Hereby, the partial Viral Protein 1 sequences of Coxsackievirus B5 (CV-B5) from sewage samples, collected in Italy from 2016 to 2017, were compared with those available in GenBank from clinical samples. Phylogenetic analysis highlighted: (I) the predominant circulation of CV-B5 genogroup B in Italy, and (II) the presence of two new sub-genogroups.

Keywords: CV-B5; Coxsackievirus; Non-polio enteroviruses; Phylogenetic analysis; Polioviruses; Sewage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enterovirus B, Human / classification
Enterovirus B, Human / genetics*
Enterovirus B, Human / isolation & purification
Environmental Monitoring
Italy
Phylogeny
Sewage / virology*
Viral Proteins / genetics
Viral Proteins / metabolism

Substances

Sewage
Viral Proteins