Serum soluble Fas ligand levels and peripheral blood lymphocyte subsets in patients with drug-induced maculopapular rashes, dress, and viral exanthemas

Background: Fatty acid synthetase (Fas)/Fas ligand (FasL)-dependent apoptotic pathways have been reported as being involved in the pathogenesis of drug-induced maculopapular rashes.

Objective: We investigated serum soluble FasL (sFasL) levels and peripheral blood lymphocyte subtypes to discriminate maculopapular drug eruptions (MPDE) from viral exanthema (VE).

Patients/methods: Children with confirmed MPDE (group I), VE (group II), and drug rashes with eosinophilia and systemic symptoms (DRESS) or drug-induced hypersensitivity syndrome (DIHS) (group III) were included. Serum sFasL levels and peripheral blood lymphocyte subtypes were analyzed in groups I-III on admission, and repeated twice (only once for group IV - controls).

Results: There were no significant serum soluble FasL level differences among the groups for all the samples. In the initial samples, CD19⁺ cell numbers in group II were significantly higher than in group IV, and the CD4⁺/CD8⁺ ratio was higher than groups I and IV. In the second samples, CD4⁺ and CD19⁺ cell numbers were significantly higher in group II than group I. In the final samples, CD4⁺ cell numbers in group II were significantly higher than group I and group III. CD19⁺ cells numbers in group III were significantly lower than the other groups for all samples.

Conclusion: Serum sFasL levels were not found to be useful in discriminating viral exanthemas from other drug rashes. The significant differences between MPDE, VE, and DRESS were high CD4⁺ and CD19⁺ cell-count numbers in VE but low B-cell numbers in DRESS. This might be important for discriminating VE from DRESS, and the low B-cell count in early symptoms might be a useful predictor of DRESS development.