Abstract
The role proteoglycans play in tumor formation was examined by measuring the tumorigenicity of proteoglycan-deficient Chinese hamster ovary cell mutants in nude mice. When 10(7) cells were injected subcutaneously, mutants with less than about 15% of the wild-type level of proteoglycan synthesis did not produce tumors. Mutants defective in the synthesis of heparan sulfate proteoglycans also did not form tumors, whereas mutants with altered chondroitin sulfate proteoglycans were tumorigenic. Tumors arose from mixtures of wild-type and nontumorigenic mutant cells and contained both cell types, suggesting that wild-type cell proteoglycans enabled mutant cells to survive. The failure of heparan sulfate-deficient mutants to form tumors depended on the ability of the host to mount a B cell-mediated immune reaction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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Cell Line
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Chondroitin Sulfate Proteoglycans / genetics
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Chondroitin Sulfate Proteoglycans / physiology
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Chondroitin Sulfates / genetics
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Chondroitin Sulfates / physiology
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Cricetinae
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Heparan Sulfate Proteoglycans
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Heparitin Sulfate / genetics
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Heparitin Sulfate / physiology
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Immunologic Deficiency Syndromes / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Mutation
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Neoplasm Transplantation
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Neoplasms, Experimental / etiology*
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Pentosyltransferases / genetics
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Pentosyltransferases / metabolism
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Proteoglycans / physiology*
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UDP Xylose-Protein Xylosyltransferase
Substances
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Chondroitin Sulfate Proteoglycans
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Heparan Sulfate Proteoglycans
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Proteoglycans
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Chondroitin Sulfates
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Heparitin Sulfate
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Pentosyltransferases