Proteomic Studies of Galectin-3 Expression in Human Thyroid Diseases by Immunodetection

Cancer Genomics Proteomics. 2006 Nov-Dec;3(6):389-394. Epub 2006 Nov 1.

Abstract

Galectin-3 expression in thyroid diseases was studied by 1-DE immunoblotting. Expression was markedly elevated in thyroid papillary carcinoma, compared to follicular adenoma, follicular carcinoma or non-neoplastic diseases. Galectin-3 expression was also elevated in malignant cancers of bone, breast, colon, esophagus, larynx, lung and ovary. Four cases of thyroid papillary carcinoma with metastasis gave 2-3 bands on 1-DE immunoblotting. 2-DE immunoblotting of gelectin-3 showed 3 dark spots with MW/pI 32.9/8.29, 31.0/8.40 and 30.0/8.40 and 2 light spots.

Keywords: Galectin-3; immunodetection; papillary carcinoma; proteomics; thyroid diseases.